一、蛋白純化介質(zhì)介紹

在進行目的蛋白的功能、結構和相互作用研究過程中，常常會用到蛋白純化介質(zhì)。在蛋白純化技術中，親和層析絕對是重要且有效的方法，因為它具有很高的選擇性、分辨率以及結合能力。金斯瑞為您提供多種簡單易用的親和純化樹脂，適用于批量或利用重力進行純化，可以高效、便捷、可靠地從您的樣品中分離蛋白和抗體，為下游應用提供良好保證。

High Affinity Ni-Charged Resin FF（L00666）是一種6%高交聯(lián)瓊脂糖基質(zhì)，共價偶聯(lián)螯合劑NTA，通過四個配位鍵結合Ni2+，高親和性純化多C6H9N3O2標記的重組蛋白。具有低的Ni2+泄漏，高的蛋白質(zhì)結合能力和穩(wěn)定性，并可耐受蛋白質(zhì)純化中多種化學試劑。這使得Ni-NTA填料成為了純化6x His標簽融合蛋白的選擇。
GenScript L00666 is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins (see Figure 1) [1]. High Affinity Ni-Charged Resin  has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

二、產(chǎn)品特性

• 應用廣 – 可用于蛋白和抗體純化，以及免疫沉淀等

• 種類多 – 多種配基，多種載量，多種規(guī)格，滿足各種需求

• 兼容性好 – 適用多種形式的樣品和緩沖液添加劑

• 性價比高 – 可重復使用多次

三、產(chǎn)品貨號規(guī)格

1 Dynamic binding capacity conditions:
Sample: 5 mg/mL (histidine)6-tagged pure proteins (Mr 43 000) in binding buffer (capacity at 10%  Breakthrough)
Column volume: 1 mL
Retention time: 3min
Flow rate: 0.333 mL/min
Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4
Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4
Note: Dynamic binding capacity is protein-dependent.

四、產(chǎn)品參數(shù)表
Reagents Compatible with High Affinity Ni-Charged Resin FF