The ToxinEraser™ Endotoxin Removal Resin utilizes immobilized polymixin B to bind and remove pyrogens from solution. The polymixins are a family of antibiotics that contain a cationic cyclopeptide with a fatty acid chain. Polymixin B neutralizes the biological activity of endotoxins by binding to the lipid A portion of bacterial lipopolysaccharide.

Description

Lipopolysaccharide (LPS) is a bacterial endotoxin, and a major constituent of the cell walls of gram-negative bacteria. During gram-negative bacteremia or endotoxemia, LPS is the principal pathophysiological mediator by which bacteria can cause hypotension, organ failure, disseminated intravascular coagulation, and fatal shock in mammalian hosts. The removal of these endotoxins is highly necessary for downstream processes.

The ToxinEraser™ Endotoxin Removal Resin utilizes immobilized polymixin B to bind and remove pyrogens from solution. The polymixins are a family of antibiotics that contain a cationic cyclopeptide with a fatty acid chain. Polymixin B neutralizes the biological activity of endotoxins by binding to the lipid A portion of bacterial lipopolysaccharide. 

產(chǎn)品概述

Product Information

Binding Capacity： Up to 2,000,000 EU/ml resin. 

Ligand： Modified PMB (Polymixin B)

 pH Stability： pH 5 -10 

Support Matrix ：4% cross-linked agarose, spherical beads 

Mean Particle Size ：90 μm 

Types of substances that can be applied to the column ：Protein, including peptides and antibody, polysaccharide, etc. 

Applicable Ionic Strength ：0.1 to 0.5 M NaCl 

Substances tested that do not interfere with performance： 20% DMSO, 20% ethanol, 20% glycerol; 1 M urea, 300 mM imidazole; 0.05% Tween 20, 10 mM DTT, etc.

Key Features

High stability and high removal efficiency.

High binding capacity: > 2, 000, 000 EU / ml (CV).

Reusable up to five times if properly regenerated.

產(chǎn)品屬性

Storage & Stability ：2°C to 8°C (Do not freeze.)