產(chǎn)品簡介Expression SystemE.coliTagTag-freeSpeciesSerratia MarcescenBiological Activity≥ 1.1×106 U/mgEnzyme Activity≥ 250 U/μLUnit DefinitionOne unit of Benz-Neburase, tag-free is defined as the amount of enzyme for a ?A260 of 1.0 (equivalent to the complete digestion of 37 μg DNA) in 30 min.Purity≥ 95% as analyzed by reducing SDS-PAGEEndotoxin Level≤ 0.1 EU/kU as determined by gel clotting methodTheoretical Molecular Weight27.5 kDaApparent Molecular Weight~27.5 kDa, on SDS-PAGE under reducing conditionsFormulationSupplied as a solution of 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0ApplicationThe Benz-Neburase™ is an endonuclease capable of removing all forms of DNA and RNA, including double stranded, single stranded, linearized, and circular forms. The Benz-Neburase is commonly used in biopharmaceutical production such as vaccine, viral vector, gene and cell therapy manufacturing facilities.Notes: The activity of Benz-Neburase™, tag-free requires 1-2 mM Mg2+.Storage & StabilityThis product remains stable for 2 weeks at 4 °C or 24 months at - 20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C!